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Create visium-with-probes-v3.1.yaml
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Removed lab_processed\/images\/[^\/]+\.json
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j-uranic committed Aug 29, 2024
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files:
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pattern: extras\/.*
required: True
description: Folder for general lab-specific files related to the dataset
-
pattern: extras\/microscope_hardware\.json
required: True
description: A file generated by the micro-meta app that contains a description of the hardware components of the microscope. Email HuBMAP Consortium Help Desk <[email protected]> if help is required in generating this document.
is_qa_qc: True
-
pattern: extras\/microscope_settings\.json
required: False
description: A file generated by the micro-meta app that contains a description of the settings that were used to acquire the image data. Email HuBMAP Consortium Help Desk <[email protected]> if help is required in generating this document.
is_qa_qc: True
-
pattern: raw\/.*
required: True
description: All raw data files for the experiment.
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pattern: raw\/[^\/]+\.gpr
required: True
description: This is a 10X Genomics layout file that's generated by 10X and individualized for each Visium slide. This is a text file and can be generated using this 10X web form <https://support.10xgenomics.com/spatial-gene-expression/software/pipelines/latest/using/slidefile-download> along with the unique 10X Visium slide ID.
is_qa_qc: False
-
pattern: raw\/additional_panels_used\.csv
required: False
description: If multiple commercial probe panels were used, then the primary probe panel should be selected in the "oligo_probe_panel" metadata field. The additional panels must be included in this file. Each panel record should include:manufacturer, model/name, product code.
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pattern: raw\/custom_probe_set\.csv
required: False
description: This file should contain any custom probes used and must be included if the metadata field "is_custom_probes_used" is "Yes". The file should minimally include:target gene id, probe seq, probe id. The contents of this file are modeled after the 10x Genomics probe set file (see <https://support.10xgenomics.com/spatial-gene-expression-ffpe/probe-sets/probe-set-file-descriptions/probe-set-file-descriptions#probe_set_csv_file>).
-
pattern: raw\/fastq\/.*
required: True
description: Raw sequencing files for the experiment
-
pattern: raw\/fastq\/oligo\/
required: True
description: Directory containing fastq files pertaining to oligo sequencing.
-
pattern: raw\/fastq\/oligo\/[^\/]+\.fastq\.gz
required: True
description: This is a gzip version of the fastq file. This file contains the cell barcode and unique molecular identifier (technical).
is_qa_qc: False
-
pattern: raw\/images\/.*
required: True
description: Directory containing raw image files. This directory should include at least one raw file.
-
pattern: raw\/images\/[^\/]+_tissue\.(?:tif|tiff)
required: False
description: Raw microscope file for the experiment. For 10X Visium CytAssist, this would be the high resolution image produced.
is_qa_qc: False
-
pattern: raw\/images\/[^\/]+_fiducial\.(?:tif|tiff)
required: True
description: This is the low resolution image from the 10X CytAssist instrument that includes the fiduciary markings.
-
pattern: raw\/images\/[^\/]+\.ndpi
required: False
description: Raw microscope file for the experiment
is_qa_qc: False
-
pattern: lab_processed\/.*
required: True
description: Experiment files that were processed by the lab generating the data.
-
pattern: lab_processed\/alignment\.json
required: True
description: JSON file for the manual tissue alignment created using Loupe browser and used as input to Space Ranger.
-
pattern: lab_processed\/images\/.*
required: True
description: Processed image files
-
pattern: lab_processed\/images\/[^\/]+\.ome\.tiff
required: True
description: OME-TIFF files (multichannel, multi-layered) produced by the microscopy experiment. If compressed, must use loss-less compression algorithm. For Visium this stitched file should only include the single capture area relevant to the current dataset. For GeoMx there will be one OME TIFF file per slide, with each slide including multiple AOIs. See the following link for the set of fields that are required in the OME TIFF file XML header. <https://docs.google.com/spreadsheets/d/1YnmdTAA0Z9MKN3OjR3Sca8pz-LNQll91wdQoRPSP6Q4/edit#gid=0>
is_qa_qc: False
example: lab_processed/images/HBM892.MDXS.293.ome.tiff
-
pattern: lab_processed\/images\/[^\/]*ome-tiff\.channels\.csv
required: True
description: This file provides essential documentation pertaining to each channel of the accommpanying OME TIFF. The file should contain one row per OME TIFF channel. The required fields are detailed <https://docs.google.com/spreadsheets/d/1xEJSb0xn5C5fB3k62pj1CyHNybpt4-YtvUs5SUMS44o/edit#gid=0>
is_qa_qc: False

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