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lhao03 committed Apr 4, 2024
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- [Overview](./wet-lab/index.md)
- [Notebook](./wet-lab/notebook/index.md)
- [March](./wet-lab/notebook/march.md)
- [April](./wet-lab/notebook/april.md)
- [Design](./wet-lab/design/index.md)
- [Solid phase DNA Synthesis](./wet-lab/design/solid-phase.md)
- [ssDNA to dsDNA](./wet-lab/design/dsdna-experiment.md)
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[Meeting Notes](https://drive.google.com/drive/folders/1E_gh956eETLY7uBk27NX-Mp3P9KKm9QV?usp=sharing)

## Notebook
For each notebook entry, copy the template [here](https://github.com/UBC-iGEM/internal-wiki-2023-24/blob/main/src/wet-lab/notebook/month.md). Additionally, add a weekly summary to the [main](notebook/index.md) notebook page.
## Goals
- Work as a team through the DBTL cycle
- Practice experimental planning, analysis and data collection
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# April
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# Notebook
# Notebook: Weekly Summaries

Welcome to the Wet Lab notebook. For each month, create a new file in SUMMARY.md with the naming convention: month.md. For each day you add a journal entry, go to the corresponding month.md file, and at the top of the file, and add an entry, here's an example:
Please add a weekly summary to this page.

## May 4th, 2022

### People:
Oliver, Edward, Michaela, Anjali, Amy, Bhavya, Chloe, Shaqed, Stella

### Tasks:

- Transformation results:
- Only RFP colonies grew (Pictured) on the RFP only plate and the plasmid mix plate
![description](../images/2022-05-04/left.png)
![description](../images/2022-05-04/right.png)


- New transformations
- 2 AmilCP plates chloramphenicol LB agar plate, left in 37C room overnight

- Vibrio natriegens media preparation
- 297 mls of 300 ml prepared 2x LB + 7.04 g/l NaCl - autoclaved
- 3 mls of 4ml prepared 420 mM KCL and 2.314 M MgCl2 - filter sterilized
- 297 + 3 = 300 ml final media

### Notes:

If you need to add images, make a folder with the date with in the wet lab folder, and add images into that folder. An example already exists for the entry above. Then go to your file and add the images like this:

`
![description](../images/2022-05-04/left.png)
`

If you want to add subscripts or superscripts, or any sort of mathematical notation, please use \\[ \LaTeX \\]

`
\\[ \text{MgCl}_2 \\]
`

\\[ \text{MgCl}_2 \\]

If you don't use Latex Lucy will be very sad :(, you can search on Google how to use Latex.
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# Lab Notebook Template

Welcome to the Wet Lab notebook. For each month, create a new file in SUMMARY.md with the naming convention: month.md. For each day you add a journal entry, go to the corresponding month.md file, and at the top of the file, and add an entry, here's a template, and an example from last year:

# Experiment Title
yyyy-mm-dd

Expand All @@ -17,10 +19,50 @@ Written by:

## Results: Please attach pictures!!!!!


### Table template
| | | | | |
|---|---|---|---|---|
| | | | | |
| | | | | |
| | | | | |
| | | | | |

---

## May 4th, 2022

### People:
Oliver, Edward, Michaela, Anjali, Amy, Bhavya, Chloe, Shaqed, Stella

### Tasks:

- Transformation results:
- Only RFP colonies grew (Pictured) on the RFP only plate and the plasmid mix plate
![description](../images/2022-05-04/left.png)
![description](../images/2022-05-04/right.png)


- New transformations
- 2 AmilCP plates chloramphenicol LB agar plate, left in 37C room overnight

- Vibrio natriegens media preparation
- 297 mls of 300 ml prepared 2x LB + 7.04 g/l NaCl - autoclaved
- 3 mls of 4ml prepared 420 mM KCL and 2.314 M MgCl2 - filter sterilized
- 297 + 3 = 300 ml final media

### Notes:

If you need to add images, make a folder with the date with in the wet lab folder, and add images into that folder. An example already exists for the entry above. Then go to your file and add the images like this:

`
![description](../images/2022-05-04/left.png)
`

If you want to add subscripts or superscripts, or any sort of mathematical notation, please use \\[ \LaTeX \\]

`
\\[ \text{MgCl}_2 \\]
`

\\[ \text{MgCl}_2 \\]

If you don't use Latex Lucy will be very sad :(, you can search on Google how to use Latex.

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