Image analysis pipeline to detect exocytosis (vesicle fusion at plasma membrane) on TIRF microscopy timelapse images
This protocols is a two-step process
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The first part of the process is the automatic event detection using ImageJ and ImageJ plugin TrackMate - Event_detection_TrackMate
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The second part of the process is the filtering of false events and determination of the vesicle type, done in a Jupyter nNotebook - Event_filter_graph