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Support GAF alignment files #417

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adamnovak opened this issue Apr 9, 2024 · 2 comments
Open

Support GAF alignment files #417

adamnovak opened this issue Apr 9, 2024 · 2 comments

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@adamnovak
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Now that vg supports indexed GAF files, we should be able to support GAF alongside GAM as a file type for read tracks.

@adamnovak
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This might need the -F option to vg chunk when passing it a GAF.

@adamnovak
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To make this work, we need to:

  • Add gaf as a recognized extension:
    "read": ".gam"
  • Change a bunch of code in the server that uses "gam" to refer to the concept of a reads file:
    let gamFiles = getGams(req.body.tracks);
    console.log("graphFile ", graphFile);
    console.log("gbwtFile ", gbwtFile);
    console.log("bedFile ", bedFile);
    console.log("gamFiles ", gamFiles);
    req.withGam = true;
    if (!gamFiles || !gamFiles.length) {
    req.withGam = false;
    console.log("no gam index provided.");
    }

    // push all gam files
    for (const gamFile of gamFiles) {
    if (!gamFile.endsWith(".gam")) {
    throw new BadRequestError("GAM file doesn't end in .gam: " + gamFile);
    }
    if (!isAllowedPath(gamFile)) {
    throw new BadRequestError("GAM file path not allowed: " + gamFile);
    }
    // Use a GAM index
    console.log("pushing gam file", gamFile);
    vgChunkParams.push("-a", gamFile, "-g");
    }
  • Also in that last block, add code to use -F for GAF files. I think this might be a flag to mark the "GAM" files as actually GAF; we might really need to teach vg chunk to mix GAM and GAF files to do this properly.
  • Fix some more loactions where we explicitly say we're passing around GAM files:
    gamIndices: ["cactus-NA12879.sorted.gam"],

  • Adjust the importer scripts to allow GAF files in addition to GAM files.

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