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I’m using bcftools (v. 1.20) for variant calling with mpileup and bcftools call, but I’m encountering the following error message:
"Failed to read from standard input: unknown file type".
I’m using the -b bam_files.txt option, which includes a list of my BAM files (e.g., a.bam.gz, b.bam.gz, etc.). I suspect the issue is related to my BAM files being gzipped. Unfortunately, due to their large size, I don’t have enough space to unzip them.
Is there a way to use gzipped BAM files directly in the variant calling analysis? Any guidance would be appreciated.
Thanks!
The text was updated successfully, but these errors were encountered:
I’m using bcftools (v. 1.20) for variant calling with mpileup and bcftools call, but I’m encountering the following error message:
"Failed to read from standard input: unknown file type".
I’m using the -b bam_files.txt option, which includes a list of my BAM files (e.g., a.bam.gz, b.bam.gz, etc.). I suspect the issue is related to my BAM files being gzipped. Unfortunately, due to their large size, I don’t have enough space to unzip them.
Is there a way to use gzipped BAM files directly in the variant calling analysis? Any guidance would be appreciated.
Thanks!
The text was updated successfully, but these errors were encountered: