diff --git a/lib/modules/manager/pep621/processors/uv.ts b/lib/modules/manager/pep621/processors/uv.ts
index e59ac557dc1f3c..044e4163115ec6 100644
--- a/lib/modules/manager/pep621/processors/uv.ts
+++ b/lib/modules/manager/pep621/processors/uv.ts
@@ -1,4 +1,6 @@
 import is from '@sindresorhus/is';
+import { PypiDatasource } from '../../../datasource/pypi';
+import { find } from '../../../../util/host-rules';
 import { quote } from 'shlex';
 import { TEMPORARY_ERROR } from '../../../../constants/error-messages';
 import { logger } from '../../../../logger';
@@ -15,6 +17,7 @@ import type {
 import { type PyProject, UvLockfileSchema } from '../schema';
 import { depTypes, parseDependencyList } from '../utils';
 import type { PyProjectProcessor } from './types';
+import { HostRule } from '../../../../types';
 
 const uvUpdateCMD = 'uv lock';
 
@@ -115,8 +118,12 @@ export class UvProcessor implements PyProjectProcessor {
         constraint: config.constraints?.uv,
       };
 
+      const extraEnv = {
+        ...getUvExtraIndexUrl(updateArtifact.updatedDeps),
+      };
       const execOptions: ExecOptions = {
         cwdFile: packageFileName,
+        extraEnv,
         docker: {},
         userConfiguredEnv: config.env,
         toolConstraints: [pythonConstraint, uvConstraint],
@@ -191,3 +198,31 @@ function generateCMD(updatedDeps: Upgrade[]): string {
 
   return `${uvUpdateCMD} ${deps.map((dep) => `--upgrade-package ${quote(dep)}`).join(' ')}`;
 }
+
+function getMatchingHostRule(url: string | undefined): HostRule {
+  const scopedMatch = find({ hostType: PypiDatasource.id, url });
+  return is.nonEmptyObject(scopedMatch) ? scopedMatch : find({ url });
+}
+
+function getUvExtraIndexUrl(updatedDeps: Upgrade[]): NodeJS.ProcessEnv {
+  const registryUrls = updatedDeps.map((dep) => dep.registryUrls).flat();
+  const extraIndexUrls: string[] = [];
+
+  for (const registryUrl of registryUrls) {
+    const parsedUrl = new URL(registryUrl ?? '');
+
+    const rule = getMatchingHostRule(parsedUrl.toString());
+    if (rule.username) {
+      parsedUrl.username = rule.username;
+    }
+    if (rule.password) {
+      parsedUrl.password = rule.password;
+    }
+
+    extraIndexUrls.push(parsedUrl.toString());
+  }
+
+  return {
+    UV_EXTRA_INDEX_URL: extraIndexUrls.join(' '),
+  };
+}