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what is the Dumped_Pairs files about #648

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Hanjiangna opened this issue Sep 12, 2024 · 3 comments
Open

what is the Dumped_Pairs files about #648

Hanjiangna opened this issue Sep 12, 2024 · 3 comments

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@Hanjiangna
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Hi,
I use HiC-Pro-3.1.0 to process my Hi-C data to mount haplotype genome chromosomes , and I've noticed a high proportion of Dumped_pairs (approximately 95% of the total reads). I am confused as to why my HiC-Pro results do not include Valid_interaction_pairs. Could you help me understand this?
Valid_interaction_pairs 0
Valid_interaction_pairs_FF 0
Valid_interaction_pairs_RR 0
Valid_interaction_pairs_RF 0
Valid_interaction_pairs_FR 0
Dangling_end_pairs 0
Religation_pairs 0
Self_Cycle_pairs 0
Single-end_pairs 0
Filtered_pairs 1075688
Dumped_pairs 19635023

@nservant
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dumped_pairsare all pairs which are filtered out by the different parameters like inserts size, restriction fragments size or for which we were not able to reconstruct the ligation product

@Hanjiangna
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Hello
"How can I adjust the parameters to reduce the number of dumped_pairs?"
Thanks

@nservant
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remove the filters about insert size and restriction fragments size in the config file

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