make: *** [bowtie_pairing] Error 1

[wangguiqian]

Run HiC-Pro 3.1.0

Fri Mar 22 09:41:48 CST 2024
Bowtie2 alignment step1 ...
Logs: logs/V991/mapping_step1.log

---

Fri Mar 22 09:55:19 CST 2024
Bowtie2 alignment step2 ...
Logs: logs/V991/mapping_step2.log

---

Fri Mar 22 10:04:10 CST 2024
Combine R1/R2 alignment files ...
Logs: logs/V991/mapping_combine.log

---

Fri Mar 22 10:08:50 CST 2024
Mapping statistics for R1 and R2 tags ...
Logs: logs/V991/mapping_stats.log

---

Fri Mar 22 10:11:43 CST 2024
Pairing of R1 and R2 tags ...
Logs: logs/V991/mergeSAM.log
make: *** [bowtie_pairing] Error 1

The V991_hicpro_output/logs/V991/mergeSAM.log show:

/public/home/software/opt/bio/software/Python/3.8.6/bin/python /public/home/software/opt/bio/software/HiC-Pro/3.1.0//HiC-Pro_3.1.0/scripts/mergeSAM.py -q 10 -t -v -f bowtie_results/bwt2/V991/V991_R1_V991.bwt2merged.bam -r bowtie_results/bwt2/V991/V991_R2_V991.bwt2merged.bam -o bowtie_results/bwt2/V991/V991_V991.bwt2pairs.bam
Traceback (most recent call last):
File "/public/home/software/opt/bio/software/HiC-Pro/3.1.0//HiC-Pro_3.1.0/scripts/mergeSAM.py", line 21, in
import pysam
File "/public/home/software/opt/bio/software/HiC-Pro/3.1.0/lib/python3.8/site-packages/pysam/init.py", line 16, in
import pysam.libcsamfile as libcsamfile
File "pysam/libcsamfile.pyx", line 1, in init pysam.libcsamfile
File "pysam/libcalignedsegment.pyx", line 61, in init pysam.libcalignedsegment
File "/public/home/software/opt/bio/software/Python/3.8.6/lib/python3.8/ctypes/init.py", line 7, in
from _ctypes import Union, Structure, Array
ImportError: libffi.so.7: cannot open shared object file: No such file or directory