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Hi, I aligned the transcript to the reference genome, and based on the coverage of reads, I found two alternative splicing events. However, the GTF file expanded by IsoQuant shows only one transcript when viewed through IGV. Thank you!
The text was updated successfully, but these errors were encountered:
This two isoforms are quite hard to distinguish, since the second one is the a substing of the first one.
IsoQuant takes into account that some reads can be truncated, and thus considers reads from a shorter isoform simply as truncated versions of the longer isoform. If the difference was on the 3' end and two isoforms had distinct polyA sites, there would be a higher chance of detecting both of them.
We are working on improving the algorithms for correctly detecting 5' and 3' ends, but this case seems quite non-trivial. Using such reads in other cases may lead to a high number of false positives.
You may try using --fl_data option, but I don't think it will make a difference in this case.
Thank you for your reply. I also tried the '--fl_data' parameter today, and the number of transcripts in the GTF file is the same as when not using '--fl_data'. There is indeed no difference.
Hi, I aligned the transcript to the reference genome, and based on the coverage of reads, I found two alternative splicing events. However, the GTF file expanded by IsoQuant shows only one transcript when viewed through IGV. Thank you!
The text was updated successfully, but these errors were encountered: