v0.16.0

Bismark

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• File endings .fastq | .fq | .fastq.gz | .fq.gz are now removed from the output file (unless they were specified with --basename) in a bid to reduce the length of the already long file names.
• Enabled the new option --dovetail (which will be turned on by default for --pbat libraries) which will now allow dovetailing reads to be reported. For a more in-depth description see #14.
• Changed the behaviour of corner cases to where several non-directional alignments could have existed for the very same position but to different strands so that now the best alignment trumps the weaker one. As an example: If you relaxed the alignment criteria of a given alignment to allow ~60 mismatches for PE alignment we did find an alignment to the OT strand with a combined AS of -324, but there also was an alignment to the CTOB strand with and AS of 0 (perfect alignment). The CTOB now trumps the OT alignment, and the methylation information information is now reported for the bottom strand. Credits go to Sylvain Foret (ANU, Canberra) for bringing this to our attention!

New module: bismark2summary

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• bismark2summary accepts Bismark BAM files as input. It will then try to identify Bismark reports, and optionally deduplication reports or methylation extractor (splitting) reports automatically based the BAM file basename. It produces a tab delimited overview table (.txt) as well as a graphical HTML report.
  Examples can be found at http://www.bioinformatics.babraham.ac.uk/projects/bismark/bismark_summary_report.html and http://www.bioinformatics.babraham.ac.uk/projects/bismark/bismark_summary_report.txt. Thanks to @ewels for help with the Java Script part!

New module: bam2nuc

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• The new Bismark module bam2nuc calculcates the average mono- and di-nucleotide coverage of libraries and compares this to the genomic average composition. bam2nuc can be called straight from within Bismark (option --nucleotide_coverage) or run stand-alone. bam2nuc creates a ...nucleotide_stats.txt file that is also automatically detected by bismark2report and incorporated into the HTML report.
  

bismark2_sitrep.tpl

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• Removed an extra function call in bismark_sitrep.tpl so that the M-bias 2 plot is drawn once the M-bias 1 plot has finished drawing (parallel processing could with certain browsers and data may have resulted in a white spaceholder only).

methylation extractor

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• Altering the file path handling of coverage2cytosine and bismark2bedGraph also required some changes in the methylation extractor.

bismark2bedGraph

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• Input file path handling has been completely reworked. The output file which can be specified as -o output.bedGraph now has to be a single file name and mustn't contain any path information. A particular output folder may be specified with -dir /any/path/.
• Addressing the file path handling issue also fixed a similar issue with the option --remove_spaces when -o had been specified.

coverage2cytosine

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• Changed zcat for gunzip -c when reading a gzipped coverage file. This should avoid some Mac platforms crashing because zcat invariably requires a file to end in the .Z (which it doesn't...)
• Changed the way in which the coverage input file is handed over from the methylation_extractor
  to coverage2cytosine (previously the path information might have been part of the file name, but
  instead it will now be only part of the -dir output_directory option.